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SKU(재고 관리 코드):LMA819Hu
Magnetic Luminex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc.
Magnetic Luminex Assay Kit for Parathyroid Hormone Related Protein (PTHrP) ,etc.
Product No.
LMA819Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.98-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone Related Protein (PTHrP) ,etc..
No significant cross-reactivity or interference between Parathyroid Hormone Related Protein (PTHrP) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Parathyroid Hormone Related Protein (PTHrP) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Parathyroid Hormone Related Protein (PTHrP) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-105 | 101 |
| EDTA plasma(n=5) | 79-89 | 85 |
| heparin plasma(n=5) | 81-98 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone Related Protein (PTHrP) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone Related Protein (PTHrP) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Parathyroid Hormone Related Protein (PTHrP) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-101% | 94-103% | 80-91% | 85-93% |
| EDTA plasma(n=5) | 98-105% | 99-105% | 80-105% | 78-91% |
| heparin plasma(n=5) | 78-94% | 84-101% | 78-99% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:PTHrP) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Clinical and Experimental Metastasis | Characterization of a new renal cell carcinoma bone metastasis mouse model PubMed: 20443133 |
| Stem Cells and Development | Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. PubMed: 22133004 |
| International journal of biological sciences | PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition PubMed: PMC3753447 |
| Investigational New Drugs | Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts Pubmed: 23673814 |
| Toxicology and Applied Pharmacology | Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment Pubmed:24463094 |
| Arch Gynecol Obstet. | Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study Pubmed:24943060 |
| PLoS One | 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss PubMed: 25859665 |
| Endocrinology | A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis PubMed: 26135836 |
| Tumour Biol | Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. Pubmed:27312487 |
| 35 | Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin pubmed:27740628 |
| Bone | Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone Pubmed:29382610 |
| International Journal of Molecular Sciences | A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice Pubmed: 31600911 |
| Journal of Orthopaedic Surgery and Research | Identification of a potential gene target for osteoarthritis based on bioinformatics analyses Pubmed: 32571421 |
| International Immunopharmacology | Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related … |
| Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни | |
| Cell Death Dis | Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡ 33510128 |
| Sci Rep | Effects of whole-body vibration training in a cachectic C26 mouse model 34732809 |
| Acta Physiologica | The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals Pubmed:35334154 |
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