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SKU(재고 관리 코드):LMA043Hu
Magnetic Luminex Assay Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc.
Magnetic Luminex Assay Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc.
Product No.
LMA043Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc..
No significant cross-reactivity or interference between Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-91 | 86 |
| EDTA plasma(n=5) | 82-104 | 85 |
| heparin plasma(n=5) | 84-99 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Cell Line Derived Neurotrophic Factor (GDNF) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 87-101% | 91-101% | 90-103% |
| EDTA plasma(n=5) | 78-103% | 93-102% | 79-92% | 84-101% |
| heparin plasma(n=5) | 83-101% | 89-102% | 80-105% | 95-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GDNF) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Molecular Neurobiology | Efficient Generation of Functionally Active Spinal Cord Neurons from Spermatogonial Stem Cells pubmed:27566610 |
| Chinese Medicial Journal | Safflower Extract and Aceglutamide Injection Promoting Recovery of Peripheral Innervations via Vascular Endothelial Growth Factor-B Signaling in Diabetic Mice PMC5717862 |
| The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg | Acupuncture Alters Pro-infl ammatory Cytokines in the Plasma of Maternally Separated Rat Pups pubmed:28986807 |
| Heroin Addiction And Related Clinical Problems | GDNF serum levels are found to be lower in opioid-maintained patients ISSN 2531-4122 |
| Journal of Environmental Research and Public Health | Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats Pubmed: 30974849 |
| Frontiers in Aging Neuroscience | The Effects of Nordic Walking With Poles With an Integrated Resistance Shock Absorber on Cognitive Abilities and Cardiopulmonary Efficiency in … Pubmed: 33192480 |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
| Int J Biol Macromol | The effect of biodegradable silk fibroin-based scaffolds containing glial cell line-derived neurotrophic factor (GDNF) on the corneal regeneration process 34119551 |
| Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway Pubmed:35366745 |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |
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