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SKU(재고 관리 코드):LMB231Rb
Magnetic Luminex Assay Kit for Cytokeratin 18 (CK18) ,etc.
Magnetic Luminex Assay Kit for Cytokeratin 18 (CK18) ,etc.
Product No.
LMB231Rb
Organism Species
Oryctolagus cuniculus (Rabbit).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Cytokeratin 18 (CK18) ,etc..
No significant cross-reactivity or interference between Cytokeratin 18 (CK18) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cytokeratin 18 (CK18) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Cytokeratin 18 (CK18) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 94-102 | 98 |
| heparin plasma(n=5) | 95-105 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 18 (CK18) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 18 (CK18) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytokeratin 18 (CK18) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-98% | 92-99% | 96-103% | 94-101% |
| EDTA plasma(n=5) | 99-105% | 79-89% | 86-94% | 78-101% |
| heparin plasma(n=5) | 84-95% | 88-105% | 98-105% | 78-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CK18) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Can J Physiol Pharmacol. | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis Pubmed:27186801 |
| J Alzheimers Dis | Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's Disease Pubmed:26836183 |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring |
| Biomed Chromatogr | Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD 34727379 |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074 |
| Catalog No. | Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species | Applications (RESEARCH USE ONLY!) |
| SEB231Rb | ELISA Kit for Cytokeratin 18 (CK18) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB231Rb | Magnetic Luminex Assay Kit for Cytokeratin 18 (CK18) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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