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SKU(재고 관리 코드):LMA234Hu
Magnetic Luminex Assay Kit for Immunoglobulin G4 (IgG4) ,etc.
Magnetic Luminex Assay Kit for Immunoglobulin G4 (IgG4) ,etc.
Product No.
LMA234Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G4 (IgG4) ,etc..
No significant cross-reactivity or interference between Immunoglobulin G4 (IgG4) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Immunoglobulin G4 (IgG4) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G4 (IgG4) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-105 | 98 |
| EDTA plasma(n=5) | 91-101 | 96 |
| heparin plasma(n=5) | 88-102 | 99 |
| sodium citrate plasma(n=5) | 78-96 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G4 (IgG4) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G4 (IgG4) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G4 (IgG4) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-105% | 82-101% | 97-104% | 91-103% |
| EDTA plasma(n=5) | 86-97% | 87-102% | 86-95% | 90-103% |
| heparin plasma(n=5) | 78-93% | 89-103% | 79-88% | 96-105% |
| sodium citrate plasma(n=5) | 88-104% | 90-104% | 82-99% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:IgG4) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source |
| Adv Med Sci. | Utility of serum IgG, IgG4 and carbonic anhydrase II antibodies in distinguishing autoimmune pancreatitis from pancreatic cancer and chronic pancreatitis Pubmed:25194335 |
| Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861 |
| Iranian Journal of Pharmaceutical Research | Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells Pubmed:25561924 |
| Ann Hematol | Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s disease PubMed: 26377996 |
| Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394 |
| 网页进不去，NCBI上搜不到 | Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patients article:30785 |
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