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Magnetic Luminex Assay Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc.
Magnetic Luminex Assay Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc.
Product No.
LMM285Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc..
No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-102 | 98 |
| EDTA plasma(n=5) | 94-102 | 98 |
| heparin plasma(n=5) | 84-101 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-93% | 94-101% | 78-89% | 96-103% |
| EDTA plasma(n=5) | 88-102% | 86-102% | 95-104% | 88-103% |
| heparin plasma(n=5) | 80-94% | 80-101% | 95-105% | 78-90% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SRD5a2) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Evid Based Complement Alternat Med. | Seoritae extract reduces prostate weight and suppresses prostate cell proliferation in a rat model of benign prostate hyperplasia Pubmed:24701239 |
| Neuropharmacology. | TSPO activation modulates the effects of high pressure in a rat ex vivo glaucoma model. pubmed:27596950 |
| Journal of Pharmacognosy and Phytochemistry | Effects of ethanolic extracts of Cola millenii K. Schum seed on biochemical and toxicological indices of male wistar albino rats 1616357 |
| Nutrition Research and Practice | Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats 10.4162:nrp.2018.12.e36 |
| Eur J Pharmacol | Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2 34265299 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEM285Mu | ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMM285Mu | Magnetic Luminex Assay Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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