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SKU(재고 관리 코드):LMC201Hu

Magnetic Luminex Assay Kit for Catenin Alpha 1 (CTNNa1) ,etc.

Magnetic Luminex Assay Kit for Catenin Alpha 1 (CTNNa1) ,etc.

Detection range
Size
Sensitivity

Product No.

LMC201Hu

Organism Species

Homo sapiens (Human).

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3.5h

Detection Range

0.01-10ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.003 ng/mL.

UOM

1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex


    Specificity

    This assay has high sensitivity and excellent specificity for detection of Catenin Alpha 1 (CTNNa1) ,etc..
    No significant cross-reactivity or interference between Catenin Alpha 1 (CTNNa1) ,etc. and analogues was observed.

    Recovery

    Matrices listed below were spiked with certain level of recombinant Catenin Alpha 1 (CTNNa1) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Catenin Alpha 1 (CTNNa1) ,etc. in samples.

    Matrix Recovery range (%) Average(%)
    serum(n=5) 79-97 79
    EDTA plasma(n=5) 95-103 101
    heparin plasma(n=5) 79-105 82

    Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) ,etc. were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) ,etc. were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Catenin Alpha 1 (CTNNa1) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample 1:2 1:4 1:8 1:16
    serum(n=5) 82-101% 80-93% 84-99% 96-104%
    EDTA plasma(n=5) 88-97% 94-103% 81-89% 82-104%
    heparin plasma(n=5) 84-98% 86-99% 78-98% 82-99%

    Stability

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Reagents and materials provided

    Reagents Quantity Reagents Quantity
    96-well plate 1 Plate sealer for 96 wells 4
    Pre-Mixed Standard 2 Standard Diluent 1×20mL
    Pre-Mixed Magnetic beads (22#:CTNNa1) 1 Analysis buffer 1×20mL
    Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
    Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
    Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
    Instruction manual 1

    Assay procedure summary

    1. Preparation of standards, reagents and samples before the experiment;
    2. Add 100μL standard or sample to each well,
        add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
    3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
    4. Wash plate on magnetic frame for three times;
    5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
    6. Wash plate on magnetic frame for three times;
    7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

    Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
    RPC201Hu01 Recombinant Catenin Alpha 1 (CTNNa1) Positive Control; Immunogen; SDS-PAGE; WB.
    PAC201Hu01 Polyclonal Antibody to Catenin Alpha 1 (CTNNa1) WB,IHC,ICC/IF
    MAC201Hu21 Monoclonal Antibody to Catenin Alpha 1 (CTNNa1) WB; IHC; ICC; IP.
    SEC201Hu ELISA Kit for Catenin Alpha 1 (CTNNa1) Enzyme-linked immunosorbent assay for Antigen Detection.
    LMC201Hu Magnetic Luminex Assay Kit for Catenin Alpha 1 (CTNNa1) ,etc. Magnetic Luminex Assay for Antigen Detection.
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