Uncoated Human IL-4(Interleukin 4) ELISA Kit

The UNCOATED HUMAN IL-4 ELISA KIT is a highly sensitive and specific assay designed for the quantitative determination of human interleukin 4 (IL-4) in serum, plasma, and cell culture supernatants. This kit is uncoated, meaning that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and materials for the assay, including pre-coated standards, detection antibody, streptavidin-HRP, and TMB substrate.

The UNCOATED HUMAN IL-4 ELISA KIT is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, in which a capture antibody is immobilized onto the microplate and binds to the target protein. The detection antibody, conjugated to horseradish peroxidase (HRP), is then added and binds to a different epitope on the target protein. The addition of substrate results in a colorimetric reaction, which is proportional to the amount of target protein present in the sample. The kit has a detection range of 7.8-500 pg/mL and a sensitivity of 2.0 pg/mL.

The UNCOATED HUMAN IL-4 ELISA KIT is a reliable and accurate tool for the quantification of human IL-4 in various sample types. IL-4 is a cytokine that plays a critical role in the regulation of immune responses, including the differentiation and activation of T helper 2 (Th2) cells, which are involved in allergic and autoimmune diseases. The ability to accurately measure IL-4 levels in biological samples is important for understanding the role of this cytokine in disease pathogenesis and for the development of targeted therapies. The UNCOATED HUMAN IL-4 ELISA KIT provides researchers with a sensitive and specific assay for the quantification of human IL-4, allowing for the accurate measurement of this important cytokine in a variety of sample types.