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SKU(재고 관리 코드):HEA816Hu
High Sensitive ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)
High Sensitive ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
HEA816Hu
Organism Species
Homo sapiens (Human).
Sample Type
serum, plasma and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
1.25-80ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.40ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of High Sensitive Alpha-1-Acid Glycoprotein (a1AGP).
No significant cross-reactivity or interference between High Sensitive Alpha-1-Acid Glycoprotein (a1AGP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Alpha-1-Acid Glycoprotein (a1AGP) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Alpha-1-Acid Glycoprotein (a1AGP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 86-101 | 92 |
| heparin plasma(n=5) | 80-95 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Alpha-1-Acid Glycoprotein (a1AGP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Alpha-1-Acid Glycoprotein (a1AGP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Alpha-1-Acid Glycoprotein (a1AGP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-98% | 79-90% | 87-94% | 96-103% |
| EDTA plasma(n=5) | 83-97% | 98-105% | 97-104% | 87-96% |
| heparin plasma(n=5) | 98-105% | 92-101% | 80-104% | 95-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| J Reprod Dev.? | An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro Pubmed:Pmc4219990 |
| Molecular Reproduction and Development | Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitro Pubmed:25123565 |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121 |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study PubMed: 25955845 |
| Sci Rep. | S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine pmc:PMC4810364 |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858 |
| Scientific Reports | An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle pubmed:28667317 |
| 33 | Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. pubmed:28927749 |
| Brazilian Journal of Otorhinolaryngology | Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndrome Pubmed: 30213594 |
| BioRxiv | Proteome-wide characterization and biomarker identification of intracranial aneurysm |
| The FEBS journal | Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancer Pubmed: 31482685 |
| PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken Pubmed: 32191767 |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … |
| A New Biomarker Tool for Risk Stratification in ¡°De Novo¡± Acute Heart Failure (OROME) |
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