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SKU(재고 관리 코드):IEA877Ge
Instant ELISA Kit for Thromboxane B2 (TXB2)
Instant ELISA Kit for Thromboxane B2 (TXB2)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
IEA877Ge
Organism Species
Pan-species (General).
Sample Type
Serum, plasma and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1h, 10min
Detection Range
18.52-1,500pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 7.14pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Instant Thromboxane B2 (TXB2).
No significant cross-reactivity or interference between Instant Thromboxane B2 (TXB2) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Instant Thromboxane B2 (TXB2) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Thromboxane B2 (TXB2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 78-103 | 90 |
| heparin plasma(n=5) | 79-101 | 83 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Thromboxane B2 (TXB2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Thromboxane B2 (TXB2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Thromboxane B2 (TXB2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-105% | 80-101% | 85-98% | 94-105% |
| EDTA plasma(n=5) | 93-101% | 87-95% | 85-92% | 89-96% |
| heparin plasma(n=5) | 85-103% | 89-99% | 80-101% | 90-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 5 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Share and mix. Incubate 50 minutes at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Available at SSRN 3335365 | Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers |
| journal of thoracic and cardiovascular surgery | Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers Pubmed: 32812629 |
| Thrombosis Research | Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices Pubmed: 31838449 |
| blood advances | The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis 34559203 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA877Ge11 | BSA Conjugated Thromboxane B2 (TXB2) | Immunogen; SDS-PAGE; WB. |
| MAA877Ge23 | Monoclonal Antibody to Thromboxane B2 (TXB2) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA877Ge21 | Monoclonal Antibody to Thromboxane B2 (TXB2) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA877Ge22 | Monoclonal Antibody to Thromboxane B2 (TXB2) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA877Ge | ELISA Kit for Thromboxane B2 (TXB2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA877Ge | Instant ELISA Kit for Thromboxane B2 (TXB2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA877Ge | Magnetic Luminex Assay Kit for Thromboxane B2 (TXB2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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