Cloud-clone
SKU(재고 관리 코드):SEP155Ra
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEP155Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.065ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A).
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and the recovery rates were calculated by comparing the measured value to the expected amount of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-94 | 85 |
| EDTA plasma(n=5) | 91-101 | 94 |
| heparin plasma(n=5) | 85-101 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-91% | 90-101% | 84-92% | 78-101% |
| EDTA plasma(n=5) | 80-89% | 80-101% | 92-101% | 84-91% |
| heparin plasma(n=5) | 97-105% | 83-105% | 80-89% | 78-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Medical Oncology | Wls promotes the proliferation of breast cancer cells via Wnt signaling Pubmed:25801232 |
| J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs PubMed: 26209081 |
| PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification pubmed:28380024 |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618 |
| Oxidative Medicine and Cellular Longevity | Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle Pubmed:30151071 |
| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer Doi: 10.15369/sujms.30.359 |
| Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma Pubmed: 31737122 |
| CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection Pubmed: 32920199 |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties 34823885 |
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