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SKU(재고 관리 코드):CEA171Mu

ELISA Kit for Meprin A Alpha (MEP1a)

ELISA Kit for Meprin A Alpha (MEP1a)

UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

CEA171Mu

Organism Species

Mus musculus (Mouse).

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Competitive Inhibition

Assay Length

2h

Detection Range

49.4-4,000pg/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 19.1pg/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Meprin A Alpha (MEP1a).
No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Meprin A Alpha (MEP1a) and the recovery rates were calculated by comparing the measured value to the expected amount of Meprin A Alpha (MEP1a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-95 89
EDTA plasma(n=5) 94-102 98
heparin plasma(n=5) 95-105 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Meprin A Alpha (MEP1a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-102% 78-95% 83-98% 94-102%
EDTA plasma(n=5) 91-99% 79-99% 98-105% 83-99%
heparin plasma(n=5) 99-105% 78-101% 97-105% 91-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Magazine Citations
international journal of molecular sciences Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. pubmed:28272338
血清meprin-α 在儿童川崎病诊断中的临床应用价值
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA171Mu01 Recombinant Meprin A Alpha (MEP1a) Positive Control; Immunogen; SDS-PAGE; WB.
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PAA171Mu01 Polyclonal Antibody to Meprin A Alpha (MEP1a) WB; IHC; ICC; IP.
PAA171Mu08 Polyclonal Antibody to Meprin A Alpha (MEP1a) WB; IHC; ICC; IP.
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CEA171Mu ELISA Kit for Meprin A Alpha (MEP1a) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA171Mu11 ELISA Kit DIY Materials for Meprin A Alpha (MEP1a) Main materials for "Do It (ELISA Kit) Yourself".
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