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SKU(재고 관리 코드):CEA806Eq
ELISA Kit for Beta-Endorphin (bEP)
ELISA Kit for Beta-Endorphin (bEP)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
CEA806Eq
Organism Species
Equus caballus; Equine (Horse).
Sample Type
serum, plasma, cerebrospinal fluid and other biological fluids
Test Method
Competitive Inhibition
Assay Length
2h
Detection Range
12.35-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.03pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP).
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Beta-Endorphin (bEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Endorphin (bEP) in samples.
|
Matrix |
Recovery range (%) |
Average(%) |
|
serum(n=5) |
84-101 |
87 |
|
EDTA plasma(n=5) |
82-96 |
90 |
|
heparin plasma(n=5) |
98-105 |
102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Endorphin (bEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
82-101% |
94-104% |
81-89% |
91-105% |
|
EDTA plasma(n=5) |
78-91% |
80-95% |
80-102% |
95-104% |
|
heparin plasma(n=5) |
85-94% |
85-103% |
96-103% |
82-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|
Reagents |
Quantity |
Reagents |
Quantity |
|
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
|
Standard |
2 |
Standard Diluent |
1×20mL |
|
Detection Reagent A |
1 |
Assay Diluent A |
1×12mL |
|
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
|
Reagent Diluent |
1×300µL |
Stop Solution |
1×6mL |
|
TMB Substrate |
1×9mL |
Instruction manual |
1 |
|
Wash Buffer (30 × concentrate) |
1×20mL |
Assay procedure summary
-
Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|
Magazine |
Citations |
|
Experimental Dermatology |
Endogenous μ-opioid peptides modulate immune response towards malignant melanoma Publish: RD10279 |
|
European Journal of Physiology |
Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave SpringerLink: tv015l3126833315 |
|
International Journal of Pharmaceutical Science Invention ISSN(Online) |
Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage Ijpsi: Source |
|
Neurological Sciences |
Evaluation of immune parameters in chronic migraine with medication overuse Pubmed:24867859 |
|
Brain Behav. |
Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. pubmed:28413714 |
|
Journal of Cosmet Dermatol. |
Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349 |
|
International Journal of Nursing Practice |
Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial Pubmed:29512230 |
|
КОРРЕКЦИЯ ЦИТОКИНОВОГО И ГОРМОНАЛЬНОГО ДИСБАЛАНСА ПРИ ЛЕЧЕНИИ СТАБИЛЬНОЙ СТЕНОКАРДИИ НАПРЯЖЕНИЯ 10.25207:1608-6228-2018-25-3-51-55 |
|
|
Journal of Pediatric Neurosciences |
Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria |
|
Chinese Journal of Physiology |
Blood dopamine level enhanced by caffeine in men after treadmill running Pubmed: 31793465 |
|
Free Radical Biology and Medicine |
Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in … Pubmed: 31553937 |
|
Journal of Sexual Medicine |
Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives Pubmed: 32044259 |
|
JOURNAL OF AFFECTIVE DISORDERS |
Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury Pubmed: 32961416 |
|
EUROPEAN NEUROPSYCHOPHARMACOLOGY |
Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study Pubmed: 32855024 |
|
J Burn Care Res |
Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats 34309681 |
|
European Journal of Integrative Medicine |
Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized … |
|
Catalog No. |
Related products for research use of Equus caballus; Equine (Horse) Organism species |
Applications (RESEARCH USE ONLY!) |
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|
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