Cloud-clone
SKU(재고 관리 코드):SEA222Rb
ELISA Kit for Interferon Beta (IFNb)
ELISA Kit for Interferon Beta (IFNb)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEA222Rb
Organism Species
Oryctolagus cuniculus (Rabbit).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
15.6-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 6.0pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb).
No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interferon Beta (IFNb) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Beta (IFNb) in samples.
|
Matrix |
Recovery range (%) |
Average(%) |
|
serum(n=5) |
91-102 |
95 |
|
EDTA plasma(n=5) |
91-99 |
95 |
|
heparin plasma(n=5) |
80-101 |
89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Beta (IFNb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
92-105% |
79-98% |
93-105% |
94-105% |
|
EDTA plasma(n=5) |
83-92% |
86-101% |
90-98% |
89-97% |
|
heparin plasma(n=5) |
84-99% |
79-101% |
90-98% |
86-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|
Reagents |
Quantity |
Reagents |
Quantity |
|
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
|
Standard |
2 |
Standard Diluent |
1×20mL |
|
Detection Reagent A |
1×120µL |
Assay Diluent A |
1×12mL |
|
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
|
TMB Substrate |
1×9mL |
Stop Solution |
1×6mL |
|
Wash Buffer (30 × concentrate) |
1×20mL |
Instruction manual |
1 |
Assay procedure summary
-
Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|
Magazine |
Citations |
|
20 |
Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway PubMed: 21355876 |
|
Journal of General Virology |
Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. Sgm: 9182080 |
|
Biochemical and Biophysical Research Communications |
Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage ScienceDirect: S0006291X09019822 |
|
PLoS One |
Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys PubMed: PMC3757038 |
|
PLoS Neglected Tropical Diseases |
Innate Immune Response to Rift Valley Fever Virus in Goats PubMed: PMC3335883 |
|
BioMed Research International |
Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages Pubmed: 24288685 |
|
Vet Microbiol. |
An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. Pubmed: 23850441 |
|
Food and Chemical Toxicology |
Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice Pubmed: 23535186 |
|
Veterinary immunology and immunopathology |
Experimental model of equine alveolar macrophage stimulation with TLR ligands Pubmed: 23815824 |
|
American journal of respiratory cell and molecular biology |
Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages Pubmed: 24502337 |
|
FEBS Lett. |
Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity Pubmed:24530528 |
|
Veterinary Research |
Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750 |
|
PLOS Neglected Tropical Diseases |
MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production Pubmed:25122182 |
|
Veterinary Research |
The innate immune response of equine bronchial epithelial cells is altered by training Pubmed:25595212 |
|
molecular and celluar biology |
SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity PubMed: 25939384 |
|
Arch Virol |
Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus PubMed: 25797196 |
|
J |
Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells PubMed: 26258404 |
|
Virus |
PKR activation enhances replication of classical swine fever virus in PK-15 cells PubMed: 25899421 |
|
PLoS One |
siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455 |
|
Scientific Reports |
Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 Pubmed:27090571 |
|
Front Microbiol |
Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo Pubmed:27252695 |
|
Canadian Journal of Physiology and Pharmacology |
Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. pubmed:28134560 |
|
International Journal of Molecular Medicine |
Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function pubmed:27176664 |
|
International Journal of Clinical and Experimental Pathology |
Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf |
|
Poultry Science |
ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages pubmed:27486255 |
|
Journal of Virology |
Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed: |
|
Biochemical and Biophysical Research Communications |
MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861 |
|
Virology |
The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058 |
|
EMBO Journal |
Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis Pubmed:30065070 |
|
Toxicology and applied pharmacology |
Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice Pubmed:30017639 |
|
Archives of Virology |
MAVS induces a host cell defense to inhibit CSFV infection Pubmed:29556776 |
|
Journal of virology |
Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation Pubmed: 30518647 |
|
Veterinary Immunology and Immunopathology |
Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… Doi: 10.1016/j.vetimm.2019.01.003 |
|
Science Signaling |
HIPK2 is necessary for type I interferon–mediated antiviral immunity Doi: 10.1126/scisignal.aau4604 |
|
INTERNATIONAL MEDICAL RESEARCH |
Evaluation of serum interferons in patients with age-related macular degeneration Pubmed: 31007694 |
|
Veterinary Immunology and Immunopathology |
Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens |
|
International journal of biological macromolecules |
Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction Pubmed: 31284005 |
|
Journal of virology |
Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation Pubmed: 31243133 |
|
The Journal of Latvian Academy of Sciences |
Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia |
|
Cancer Medicine |
The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 Pubmed: 31218844 |
|
FASEB Journal |
Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 |
|
PLOS Pathogens |
Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion Pubmed: 31539404 |
|
Autophagy |
Induction of autophagy and suppression of type I IFN secretion by CSFV Pubmed: 32160078 |
|
MOLECULAR IMMUNOLOGY |
BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation Pubmed: 33053462 |
|
Autophagy |
LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection Pubmed: 32924761 |
|
Arch Virol . |
Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway Pubmed: 32532134 |
|
Virulence |
Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon … |
|
bioRxiv |
Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance |
|
Res Vet Sci |
Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells 34416463 |
|
Eur J Immunol |
Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity Pubmed:35419822 |
|
ResearchSquare |
Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I |
|
Catalog No. |
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