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ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2)
ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEM285Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.061ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 2 (SRD5a2).
No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 2 (SRD5a2) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Steroid 5 Alpha Reductase 2 (SRD5a2) and the recovery rates were calculated by comparing the measured value to the expected amount of Steroid 5 Alpha Reductase 2 (SRD5a2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 88-98 | 94 |
| heparin plasma(n=5) | 98-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Steroid 5 Alpha Reductase 2 (SRD5a2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-99% | 87-102% | 95-103% | 80-94% |
| EDTA plasma(n=5) | 78-102% | 78-105% | 78-96% | 87-101% |
| heparin plasma(n=5) | 81-90% | 80-102% | 89-96% | 90-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
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| Neuropharmacology. | TSPO activation modulates the effects of high pressure in a rat ex vivo glaucoma model. pubmed:27596950 |
| Journal of Pharmacognosy and Phytochemistry | Effects of ethanolic extracts of Cola millenii K. Schum seed on biochemical and toxicological indices of male wistar albino rats 1616357 |
| Nutrition Research and Practice | Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats 10.4162:nrp.2018.12.e36 |
| Eur J Pharmacol | Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2 34265299 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEM285Mu | ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMM285Mu | Magnetic Luminex Assay Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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