Cloud-clone
SKU(재고 관리 코드):SEA014Mi
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)
ELISA Kit for Bone Morphogenetic Protein 4 (BMP4)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEA014Mi
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
31.2-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 11.7pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity for detection of Bone Morphogenetic Protein 4 (BMP4).
100% cross-reactivity of Bone Morphogenetic Protein 4 (BMP4) was observed among Human, Mouse, Rat.
Recovery
Matrices listed below were spiked with certain level of recombinant Bone Morphogenetic Protein 4 (BMP4) and the recovery rates were calculated by comparing the measured value to the expected amount of Bone Morphogenetic Protein 4 (BMP4) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-98 | 90 |
| EDTA plasma(n=5) | 89-98 | 95 |
| heparin plasma(n=5) | 90-98 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bone Morphogenetic Protein 4 (BMP4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-104% | 93-105% | 87-101% | 82-101% |
| EDTA plasma(n=5) | 78-90% | 85-93% | 93-101% | 97-105% |
| heparin plasma(n=5) | 84-102% | 83-97% | 78-101% | 79-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Journal of Cellular Biochemistry | Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway PubMed: 26681207 |
| Domestic Animal Endocrinology | Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum Pubmed:26945137 |
| FEBS Open Bio | Bone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progression Pubmed: 30972973 |
| Arch Gynecol Obstet | The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839 |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| SEA014Mi | ELISA Kit for Bone Morphogenetic Protein 4 (BMP4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA014Mi | Magnetic Luminex Assay Kit for Bone Morphogenetic Protein 4 (BMP4) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
