SKU(재고 관리 코드):SEA296Mu
ELISA Kit for Lactoperoxidase (LPO)
ELISA Kit for Lactoperoxidase (LPO)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEA296Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Breast milk, tear and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.059ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Lactoperoxidase (LPO).
No significant cross-reactivity or interference between Lactoperoxidase (LPO) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoperoxidase (LPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Magazine | Citations |
Med Sci Monit.? | The assessment of sIgA, histatin-5, and lactoperoxidase levels in saliva of adolescents with dental caries Pubmed:Pmc4087079 |
Journal of clinical periodontology | Association of salivary peroxidase activity and concentration with periodontal health–a validity study pubmed:28555944 |
Oncology Letters | Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cells Pubmed:29434943 |
Validitätsstudie zur Assoziation von Speichelperoxidase-Aktivität und-Konzentration mit der parodontalen Gesundheit | |
BRAIN RESEARCH | Cerebrospinal fluid lactoperoxidase level is enhanced in idiopathic Parkinson's disease, and correlates with levodopa equivalent daily dose 33676939 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA296Mu01 | Recombinant Lactoperoxidase (LPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA296Mu02 | Recombinant Lactoperoxidase (LPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA296Mu02 | Polyclonal Antibody to Lactoperoxidase (LPO) | WB; IHC; ICC; IP. |
PAA296Mu01 | Polyclonal Antibody to Lactoperoxidase (LPO) | WB; IHC; ICC; IP. |
LAA296Mu71 | Biotin-Linked Polyclonal Antibody to Lactoperoxidase (LPO) | WB; IHC; ICC. |
SEA296Mu | ELISA Kit for Lactoperoxidase (LPO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA296Mu | Magnetic Luminex Assay Kit for Lactoperoxidase (LPO) ,etc. | Magnetic Luminex Assay for Antigen Detection. |