SKU(재고 관리 코드):CEA218Mu
ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)
ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
CEA218Mu
Organism Species
Mus musculus (Mouse).
Sample Type
serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
2h
Detection Range
24.69-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 8.93pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 2 (TGFb2).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 2 (TGFb2) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 2 (TGFb2) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 2 (TGFb2) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 84-98 | 95 |
EDTA plasma(n=5) | 84-92 | 87 |
heparin plasma(n=5) | 93-103 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 2 (TGFb2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-88% | 92-101% | 82-94% | 81-98% |
EDTA plasma(n=5) | 90-98% | 81-103% | 96-104% | 97-105% |
heparin plasma(n=5) | 97-105% | 91-102% | 90-104% | 82-91% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
TMB Substrate | 1×9mL | Instruction manual | 1 |
Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Magazine | Citations |
international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source |
J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells PubMed: 25799173 |
International Journal of Chemical Studies | Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528 |
JCI Insight | Angiocrine signals regulate quiescence and therapy resistance in bone metastasis Pubmed: 31292293 |
Mol Med | Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma 34362313 |
Food Chem | Effect of convection and microwave heating on the retention of bioactive components in human milk 34896952 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA218Mu02 | Recombinant Transforming Growth Factor Beta 2 (TGFb2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA218Mu01 | Recombinant Transforming Growth Factor Beta 2 (TGFb2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA218Mu02 | Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) | WB; IHC; ICC; IP. |
PAA218Mu01 | Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) | WB; IHC; ICC; IP. |
SEA218Mu | ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA218Mu | Mini Samples ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA218Mu | ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA218Mu | Magnetic Luminex Assay Kit for Transforming Growth Factor Beta 2 (TGFb2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |