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SKU(재고 관리 코드):CEA218Mu

ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)

ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)

UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

CEA218Mu

Organism Species

Mus musculus (Mouse).

Sample Type

serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Test Method

Competitive Inhibition

Assay Length

2h

Detection Range

24.69-2,000pg/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 8.93pg/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 2 (TGFb2).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 2 (TGFb2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 2 (TGFb2) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 2 (TGFb2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-98 95
EDTA plasma(n=5) 84-92 87
heparin plasma(n=5) 93-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 2 (TGFb2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-88% 92-101% 82-94% 81-98%
EDTA plasma(n=5) 90-98% 81-103% 96-104% 97-105%
heparin plasma(n=5) 97-105% 91-102% 90-104% 82-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Magazine Citations
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells PubMed: 25799173
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528
JCI Insight Angiocrine signals regulate quiescence and therapy resistance in bone metastasis Pubmed: 31292293
Mol Med Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma 34362313
Food Chem Effect of convection and microwave heating on the retention of bioactive components in human milk 34896952
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA218Mu02 Recombinant Transforming Growth Factor Beta 2 (TGFb2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA218Mu01 Recombinant Transforming Growth Factor Beta 2 (TGFb2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA218Mu02 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) WB; IHC; ICC; IP.
PAA218Mu01 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) WB; IHC; ICC; IP.
SEA218Mu ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA218Mu Mini Samples ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA218Mu ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA218Mu Magnetic Luminex Assay Kit for Transforming Growth Factor Beta 2 (TGFb2) ,etc. Magnetic Luminex Assay for Antigen Detection.
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