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SKU(재고 관리 코드):CEB307Hu

ELISA Kit for Fibrinopeptide B (FPB)

ELISA Kit for Fibrinopeptide B (FPB)

UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

CEB307Hu

Organism Species

Homo sapiens (Human).

Sample Type

Serum, plasma and other biological fluids

Test Method

Competitive Inhibition

Assay Length

2h

Detection Range

370.4-30,000pg/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 133.7pg/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinopeptide B (FPB).
No significant cross-reactivity or interference between Fibrinopeptide B (FPB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinopeptide B (FPB) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinopeptide B (FPB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 89-99 95
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinopeptide B (FPB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinopeptide B (FPB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinopeptide B (FPB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 79-105% 92-101% 88-99%
EDTA plasma(n=5) 84-105% 94-103% 79-99% 78-97%
heparin plasma(n=5) 85-97% 80-99% 80-94% 89-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
CPB307Hu31 KLH Conjugated Fibrinopeptide B (FPB) Immunogen; SDS-PAGE; WB.
PAB307Hu08 Polyclonal Antibody to Fibrinopeptide B (FPB) ELISA; WB; IHC; ICC; IP.
CEB307Hu ELISA Kit for Fibrinopeptide B (FPB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB307Hu Magnetic Luminex Assay Kit for Fibrinopeptide B (FPB) ,etc. Magnetic Luminex Assay for Antigen Detection.
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