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NSJ Bioreagents
SKU(재고 관리 코드):F52067-0.08ML
Anti-His Tag Antibody
Anti-His Tag Antibody
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Application | WB, ELISA |
| Application Details | Western blot: 1:1000 |
| Application Note | Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Immunogen | HHHHHH peptide was used as the immunogen for the anti-His Tag antibody. |
| Purity | Purified |
| Storage | Aliquot the anti-His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This anti-His Tag antibody is available for research use only. |
| Status | Available |
| PDF Link | http://www.nsjbio.com/tds-pdf/anti-his-tag-antibody-f52067 |
| Title | Anti-His Tag Antibody |
| Description | Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. |
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