• Western blot analysis of lysate from 293, CEM, HeLa, Y79, rat PC-12 cell line, mouse brain tissue lysate using TUBB / beta-Tubulin antibody at 1:1000 for each lane.
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NSJ Bioreagents

F40189-0.08ML

Beta-Tubulin Antibody (TUBB)

Beta-Tubulin Antibody (TUBB)

Size

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Clone 87CT59.3.7
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, k
Species Reactivity Human, Mouse, Rat
Predicted Species Reactivity Bovine, Chicken, Hamster, Pig, Rat, Xenopus
Application WB, IHC, IF, ELISA
Application Details Western blot: 1:1000,Immunofluorescence: 1:25,IHC (Paraffin): 1:10-1:50
Application Note Titration of the beta-Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen Tubulin beta chain recombinant protein was used to produce this beta-Tubulin antibody.
Purity Purified
Storage Aliquot the beta-Tubulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This beta-Tubulin antibody is available for research use only.
Uniprot # P07437
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/beta-tubulin-antibody-tubb-87ct5937-f40189
Title Beta-Tubulin Antibody (TUBB)
Description Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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