• Flow cytometry testing of human HepG2 cells with Clathrin heavy chain antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Clathrin heavy chain antibody.
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NSJ Bioreagents

RQ6408

Clathrin heavy chain Antibody / CLTC

Clathrin heavy chain Antibody / CLTC

Size

Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Purified
Clone 6D3
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Species Reactivity Human
Application WB, IHC-P, FACS
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note Optimal dilution of the Clathrin heavy chain antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen Recombinant human protein (amino acids R967-Q1668) was used as the immunogen for the Clathrin heavy chain antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity purified
Storage After reconstitution, the Clathrin heavy chain antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Clathrin heavy chain antibody is available for research use only.
Uniprot # Q00610
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/clathrin-heavy-chain-antibody-cltc-6d3-rq6408
Title Clathrin heavy chain Antibody / CLTC
Description Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
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