• NES Antibody / Nestin, 0.4ml
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NSJ Bioreagents

F47834-0.4ML

NES Antibody / Nestin, 0.4ml

NES Antibody / Nestin, 0.4ml

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Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Specifications

Catalog No F47834-0.4ML
Family Primary antibody
Qty 0.4ml
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, IHC, IF, FACS, ELISA
Application Details Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Status Available
PDF Link Product Data Sheet (PDF)
Limitation This Nestin antibody is available for research use only.
Uniprot # P48681
Purity Antigen affinity
Immunogen A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody.
Storage Aliquot the Nestin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Localization Cytoplasmic, membranous
Title NES Antibody / Nestin
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