NDUFB11 Antibody / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, 100 ug

NDUFB11 antibody detects NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, a small integral membrane protein of the mitochondrial inner membrane essential for oxidative phosphorylation. Encoded by the NDUFB11 gene on chromosome Xp11.23, this protein is a subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), the first and largest enzyme complex of the electron transport chain. NDUFB11 contributes to electron transfer from NADH to ubiquinone, driving proton pumping and ATP synthesis. Its stability is vital for the assembly and structural integrity of Complex I, which contains over 40 subunits in mammalian cells.

Structurally, NDUFB11 is a highly conserved, hydrophobic protein of approximately 17 kilodaltons, localized to the mitochondrial inner membrane with one transmembrane helix. It anchors and stabilizes neighboring subunits within the peripheral arm of Complex I, enabling efficient electron flow and coupling of redox reactions to proton translocation. Loss or mutation of NDUFB11 disrupts complex assembly, leading to impaired respiratory chain activity and reduced ATP generation.

The NDUFB11 antibody is widely used in mitochondrial biology, metabolic disease, and bioenergetics research to study oxidative phosphorylation and Complex I organization. Western blot analysis detects a 17 kilodalton band corresponding to NDUFB11, while immunofluorescence reveals characteristic mitochondrial localization that colocalizes with markers such as COX IV. This antibody provides a reliable means of evaluating mitochondrial function, biogenesis, and assembly defects in various models.

Pathogenic variants in NDUFB11 cause X-linked mitochondrial Complex I deficiency, often presenting as hypertrophic cardiomyopathy, encephalopathy, or lactic acidosis. Altered NDUFB11 expression has also been observed in cancers and neurodegenerative disorders, where mitochondrial dysfunction contributes to disease progression. The NDUFB11 antibody enables detection of expression changes, assessment of mitochondrial health, and verification of respiratory chain assembly status. NSJ Bioreagents validates this antibody for its applications, ensuring high specificity and reproducibility for mitochondrial research.