TDRD9 Antibody / Tudor domain-containing protein 9, 100 ug

TDRD9 antibody detects Tudor domain-containing protein 9, an ATP-dependent RNA helicase that plays a crucial role in the piRNA pathway and germline genome defense. The UniProt recommended name is Tudor domain-containing protein 9 (TDRD9). This protein is a key component of the PIWI-interacting RNA (piRNA) silencing machinery, which suppresses transposable elements during spermatogenesis to maintain genomic integrity.

Functionally, TDRD9 antibody identifies a 1,278-amino-acid protein containing a C-terminal helicase domain and an N-terminal Tudor domain. The Tudor domain recognizes methylated arginine residues on PIWI proteins, while the helicase domain unwinds RNA substrates during piRNA amplification. TDRD9 forms complexes with MIWI2 and other germline-specific proteins to silence retrotransposons through DNA methylation and RNA degradation.

The TDRD9 gene is located on chromosome 14q21.1 and is expressed predominantly in male germ cells during spermatogenesis. It is localized to the cytoplasmic nuage and intermitochondrial cement structures in developing spermatocytes. TDRD9 activity ensures transposon repression and proper germ cell development.

Pathologically, mutations in TDRD9 cause non-obstructive azoospermia and male infertility due to germline arrest and failure of transposon silencing. Dysregulation of TDRD9 or the piRNA pathway may also contribute to germ cell tumors and epigenetic instability. Research using TDRD9 antibody supports studies in germline biology, RNA regulation, and epigenetic silencing.

TDRD9 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect germline helicases and piRNA pathway components. NSJ Bioreagents provides high-quality TDRD9 antibody reagents optimized for reproductive biology and RNA silencing research.

Structurally, Tudor domain-containing protein 9 contains conserved helicase motifs typical of the DEXD/H-box family and a Tudor domain that mediates protein-RNA interactions. This antibody enables investigation of TDRD9's molecular functions in piRNA biogenesis and transposon repression.