Ubiquitin Antibody, 20ug

UBB antibody recognizes Ubiquitin, a highly conserved regulatory protein encoded by the UBB gene and other ubiquitin precursor genes that is essential for intracellular protein homeostasis. Ubiquitin is a 76 amino acid polypeptide that becomes covalently attached to substrate proteins through an enzymatic cascade involving E1 activating enzymes, E2 conjugating enzymes, and E3 ligases. This post translational modification, termed ubiquitination, controls protein degradation, cellular signaling, trafficking, and stress responses.<br><br>Ubiquitin functions through the formation of mono ubiquitin modifications or polyubiquitin chains. Polyubiquitin chains linked through lysine 48 commonly target proteins for degradation by the 26S proteasome, ensuring removal of misfolded or damaged proteins and regulating turnover of key cell cycle and signaling proteins. In contrast, alternative linkages such as lysine 63 chains participate in non degradative signaling pathways including DNA damage repair, inflammatory signaling, and receptor internalization. Through these structurally distinct chain types, Ubiquitin acts as a versatile modifier that influences numerous cellular processes.<br><br>The UBB gene encodes polyubiquitin precursor proteins that are processed to generate functional ubiquitin monomers. Because ubiquitin mediated regulation is fundamental to nearly all cell types, Ubiquitin is broadly expressed in normal tissues. Disruption of ubiquitin pathways has been implicated in neurodegenerative diseases characterized by accumulation of ubiquitinated protein aggregates, as well as in cancer, where altered ubiquitin dependent degradation of tumor suppressors and oncogenic proteins contributes to malignant progression.<br><br>Ubiquitin localizes to both the cytoplasm and nucleus, reflecting its involvement in proteasomal degradation, chromatin regulation, and transcriptional control. UBB antibody can detect free ubiquitin and ubiquitin conjugated proteins depending on experimental conditions. The recombinant mouse monoclonal antibody clone rFPM1 is designed to detect Ubiquitin expression in research applications focused on proteasome biology, protein turnover, cellular stress responses, and ubiquitin signaling pathways. As a recombinant monoclonal antibody, clone rFPM1 supports consistent evaluation of UBB associated ubiquitin protein in diverse biological samples.

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