MUC16 Antibody / Cancer antigen 125, 20ug

CA125 Antibody recognizes Mucin 16 (MUC16), a very high molecular weight transmembrane glycoprotein widely known as cancer antigen 125 and ovarian cancer antigen 125. MUC16 is a membrane-associated mucin characterized by extensive O-linked glycosylation, multiple tandem repeat domains, and a large extracellular region that can be proteolytically shed into circulation. CA125 Antibody for IHC is designed for immunohistochemical detection of MUC16 expression in epithelial and tumor tissues in research applications.<br><br>MUC16 antibody, also referred to as CA125 antibody and Mucin-16 antibody in the literature, targets a mucin primarily expressed on the apical surface of epithelial cells lining the female reproductive tract, including endometrium and fallopian tube, as well as in ocular and respiratory epithelium. Under physiologic conditions, MUC16 contributes to epithelial barrier integrity, lubrication, and protection against environmental stress. Proteolytic cleavage of its extracellular domain generates circulating CA125, which is widely used as a serum biomarker for monitoring ovarian carcinoma.<br><br>The MUC16 gene is located on human chromosome 19p13.2 and encodes one of the largest membrane-associated proteins identified. Structurally, MUC16 contains numerous SEA domains, extensive tandem repeat regions, a transmembrane segment, and a short cytoplasmic tail involved in intracellular signaling interactions. MUC16 has been shown to interact with mesothelin and contribute to tumor cell adhesion and peritoneal dissemination in ovarian carcinoma models.<br><br>Aberrant overexpression of MUC16 is most strongly associated with epithelial ovarian carcinoma and is also observed in subsets of endometrial, pancreatic, breast, lung, and gastrointestinal carcinomas. In histologic sections, MUC16 typically demonstrates apical membranous and cytoplasmic staining in positive epithelial tumor cells. A CA125 antibody is commonly applied in research settings to evaluate epithelial differentiation and tumor origin. Clone CA125/9923R is a recombinant rabbit monoclonal antibody developed to target MUC16 in experimental systems.

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