His Tag Antibody, 100ug

6xHis Tag Antibody recognizes the hexahistidine sequence, also known as the His epitope tag, a short polyhistidine motif engineered into recombinant proteins for purification and detection. 6xHis Tag Antibody, also referred to as His Epitope Tag antibody, His Tag antibody, and anti-6xHis antibody in the literature, detects histidine-tagged fusion proteins expressed in bacterial, mammalian, or insect systems. Clone 6HIS/13239R is produced as a recombinant rabbit monoclonal antibody, providing defined specificity and consistent performance for detection of 6xHis-tagged recombinant proteins in research applications.<br><br>The His epitope tag typically consists of six consecutive histidine residues positioned at the N-terminus or C-terminus of a target protein. This small sequence enables purification using immobilized metal affinity chromatography and facilitates immunodetection in downstream analytical workflows. Because the 6xHis tag is compact and generally does not disrupt protein folding or biological activity, it remains one of the most widely used affinity tags in molecular biology and recombinant protein engineering.<br><br>Detection of His epitope-tagged proteins is critical for confirming expression, verifying purification efficiency, and assessing molecular integrity. Under denaturing conditions, intact fusion proteins are typically detected at their predicted molecular weight, while truncated or partially degraded forms may also be observed depending on construct design and expression system stability. Accurate recognition of the hexahistidine epitope therefore supports reliable interpretation of recombinant protein experiments.<br><br>Clone 6HIS/13239R recognizes the 6xHis sequence independently of the fused protein backbone, supporting broad detection across diverse recombinant constructs. The recombinant rabbit monoclonal format promotes lot-to-lot consistency and defined epitope recognition, enabling reproducible detection of His epitope-tagged proteins across experimental workflows.<br><br>6xHis Tag Antibody clone 6HIS/13239R is suitable for research applications focused on detection, characterization, and analysis of recombinant proteins containing a His epitope tag.

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