NSJ Bioreagents
SKU(재고 관리 코드):V6126-20UG
PGP9.5 / UchL1 Antibody, 20ug
PGP9.5 / UchL1 Antibody, 20ug
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Protein gene product 9.5 (PGP9.5), also known as Ubiquitin C-terminal hydrolase L1 (UCHL1), is a cytoplasmic protein widely used as a neuronal marker in immunohistochemistry and increasingly evaluated in tumor tissues. The PGP9.5 Antibody for IHC / UCHL1 Immunohistochemistry Antibody (clone MSVA-905R) is specifically developed for FFPE tissue staining, enabling clear visualization of UCHL1 expression patterns, cellular localization, and tissue architecture in histological sections.<br><br>PGP9.5 Antibody for IHC, also referred to as UCHL1 antibody or ubiquitin C-terminal hydrolase L1 antibody, is optimized for chromogenic immunohistochemistry rather than western blot or fluorescence-based imaging. In FFPE tissues, UCHL1 demonstrates strong cytoplasmic staining in neuronal cells, with labeling of nerve fibers and processes that highlight tissue structure. This characteristic staining pattern supports identification of neural elements and provides clear morphological context within complex tissue environments.<br><br>This recombinant rabbit monoclonal PGP9.5 Antibody for IHC (clone MSVA-905R) has been extensively evaluated using tissue microarray (TMA) panels, enabling systematic analysis across a broad range of normal and cancer tissues under standardized staining conditions. TMA-based validation allows direct comparison of staining intensity, cellular localization, and distribution patterns across many tissue types, supporting high-confidence interpretation of UCHL1 expression in both normal and pathological contexts.<br><br>In tumor tissues, UCHL1 expression can vary depending on tumor type and differentiation state, with staining observed in specific cell populations and tissue compartments. TMA analysis enables side-by-side evaluation of these patterns across multiple tumor samples, providing insight into expression variability and supporting comparative tissue profiling. The ability to assess large numbers of samples simultaneously improves consistency and reduces variability in immunohistochemical interpretation.<br><br>The PGP9.5 Antibody for IHC / UCHL1 Immunohistochemistry Antibody (clone MSVA-905R) is ideally suited for FFPE tissue staining, tumor versus normal tissue comparison, and large-scale expression profiling using TMA panels. Its positioning as a TMA-validated immunohistochemistry antibody clearly differentiates it from non-IHC reagents and supports detailed analysis of UCHL1 localization in histological studies.<br><br>This antibody is also part of a broader collection of <a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis</a>, supporting consistent staining across normal and cancer tissues.
Specifications
| Catalog No | V6126-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20ug |
| Formulation | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide |
| Format | Purified |
| Clone | MSVA-905R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Species Reactivity | Human |
| Application | IHC |
| Application Details | Immunohistochemistry (FFPE): 1:100-1:200 |
| Application Note | 1. Optimal dilution of the PGP9.5 Antibody for IHC / UCHL1 Immunohistochemistry Antibody should be determined by the researcher. <br><br>2. This PGP9.5 / UCHL1 antibody is recombinantly produced by expression in CHO cells.<br><br>3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. |
| Status | Available |
| PDF Link | Product Data Sheet (PDF) |
| Limitation | This PGP9.5 / UCHL1 antibody is available for research use only. |
| Uniprot # | P09936 |
| Immunogen | Recombinant full-length human UCHL1 protein was used as the immunogen for the PGP9.5 Antibody for IHC / UCHL1 Immunohistochemistry Antibody. |
| Storage | PGP9.5 / UCHL1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC. |
| Localization | Cytoplasm, Endoplasmic reticulum membrane |
| Title | PGP9.5 / UchL1 Antibody |