NSJ Bioreagents
SKU(재고 관리 코드):V6128-20UG
Villin Antibody, 20ug
Villin Antibody, 20ug
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Villin-1 (VIL1) is an actin-associated protein localized to the apical region of intestinal epithelial cells, where it supports brush border organization and epithelial architecture. Villin Antibody for IHC / VIL1 Immunohistochemistry Antibody (clone MSVA-459R) is specifically developed for immunohistochemistry applications, enabling consistent detection of Villin in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Villin antibody, also known as Villin-1 antibody or VIL1 antibody, is widely used in histological analysis to visualize epithelial structures and glandular morphology in gastrointestinal tissues.<br><br>This Villin Antibody for IHC is strongly differentiated by its focus on immunohistochemistry performance, FFPE compatibility, and large-scale tissue validation rather than purely biological function. The recombinant rabbit monoclonal format (clone MSVA-459R) supports high signal clarity and reproducibility, which is critical for IHC workflows. Importantly, this antibody has been evaluated using human tissue microarray (TMA) panels, demonstrating consistent staining across a broad range of normal and cancer tissues. This TMA-based validation provides confidence in staining specificity and pattern consistency in complex tissue environments.<br><br>In immunohistochemistry, Villin produces a characteristic apical and cytoplasmic staining pattern in epithelial cells, clearly outlining luminal borders and glandular structures. This makes Villin Antibody for IHC particularly valuable for assessing epithelial organization, identifying gland-forming tissues, and visualizing enterocyte-like differentiation in tumors. In colorectal and other gastrointestinal adenocarcinomas, Villin staining highlights tumor epithelial cells and supports evaluation of epithelial morphology within FFPE sections.<br><br>From a pathology and research perspective, this VIL1 Immunohistochemistry Antibody is optimized for tissue-based analysis, including FFPE sections and TMA studies, where reproducible staining and clear morphological definition are essential. Clone MSVA-459R provides a robust tool for investigators and pathologists studying epithelial architecture, tumor histology, and gastrointestinal tissue identity using immunohistochemistry.<br><br>This antibody is also part of a broader collection of <a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis</a>, supporting consistent staining across normal and cancer tissues.
Specifications
| Catalog No | V6128-20UG |
|---|---|
| Family | Primary antibody |
| Qty | 20ug |
| Formulation | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide |
| Format | Purified |
| Clone | MSVA-459R |
| Host Animal | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Species Reactivity | Human |
| Application | IHC |
| Application Details | Immunohistochemistry (FFPE): 1:100-1:200 |
| Application Note | 1. Optimal dilution of the Villin Antibody for IHC / VIL1 Immunohistochemistry Antibody should be determined by the researcher. <br><br>2. This VIL1/Villin antibody is recombinantly produced by expression in human HEK293 cells.<br><br>3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. |
| Status | Available |
| PDF Link | Product Data Sheet (PDF) |
| Limitation | This VIL1/Villin antibody is available for research use only. |
| Uniprot # | P09327 |
| Immunogen | A recombinant fragment (around amino acids 600-700) of human Villin protein (exact sequence is proprietary) was used as the immunogen for the Villin Antibody for IHC / VIL1 Immunohistochemistry Antibody. |
| Storage | VIL1/Villin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC. |
| Localization | Cell projection, Cytoplasm, Cytoskeleton, Filopodium, Filopodium tip, Lamellipodium, Microvillus, Ruffle |
| Title | Villin Antibody |