TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human p73 around the non-acetylation site of Lys321. AA range:281-330
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
70kD
GeneID（Human）:
7161
Human Swiss-Prot No:
O15350
Cellular localization:
Nucleus . Cytoplasm. Accumulates in the nucleus in response to DNA damage.
Background:
tumor protein p73(TP73) Homo sapiens This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011],
Western Blot analysis of Jurkat cells using p73 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100