Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human PAK3 around the phosphorylation site of Ser154. AA range:121-170
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
72kD
GeneID（Human）:
5063
Human Swiss-Prot No:
O75914
Cellular localization:
Cytoplasm .
Background:
The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Apr 2016],
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PAK3 (Phospho-Ser154) Antibody
Immunohistochemistry analysis of paraffin-embedded human brain, using PAK3 (Phospho-Ser154) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from rat heart, using PAK3 (Phospho-Ser154) Antibody. The lane on the right is blocked with the phospho peptide.