Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human PLD1 around the phosphorylation site of Ser561. AA range:527-576
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
GeneID（Human）:
5337
Human Swiss-Prot No:
Q13393
Cellular localization:
Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side .
Background:
This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLD1 (Phospho-Ser561) Antibody
Immunofluorescence analysis of HepG2 cells, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide.