Enzo Life Science
SKU(재고 관리 코드):ADI-KAP-PK112-F
PDK1 polyclonal antibody
PDK1 polyclonal antibody
Product Details
| Alternative Name: | Pyruvate dehydrogenase kinase |
| Host: | Rabbit |
| Immunogen: | Synthetic peptide corresponding to a portion of human PDK1 (Pyruvate dehydrogenase kinase-1). |
| UniProt ID: | Q15118 |
| GenBank ID: | L42450 |
| Source: | Purified from rabbit serum. |
| Species reactivity: | Human, Mouse, Rat Dog, Monkey, Porcine, Rabbit |
| Applications: | IHC (PS), WB |
| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~48kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | PDK-1 (Pyruvate dehydrogenase kinase-1) is a 48 kDa protein kinase. It is a member of the mitrochondrial matrix protein kinase family and is related to the histidine protein kinases found in prokaryotes. PDK-1 is responsible for phosphorylation and concomitant inactivation of pyruvate dehydrogenase. |
| Regulatory Status: | RUO - Research Use Only |
Immunohistochemistry analysis of human spleen tissue stained with PDK1, pAb at 10µg/ml.
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Product Literature References
Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression: E.S. Yang, et al.; Oncol. Rep. 49, 72 (2023), Abstract;
Inhibition of pyruvate dehydrogenase kinase 4 in CD4+ T cells ameliorates intestinal inflammation: H. Lee, et al.; Cell. Mol. Gastroenterol. Hepatol. 15, 439 (2023), Abstract;
Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma: H. Lu, et al.; JCI Insight 4, e131106 (2019), Abstract; Full Text
ASCT2 (SLC1A5) is an EGFR-associated protein that can be co-targeted by cetuximab to sensitize cancer cells to ROS-induced apoptosis: H. Lu, et al.; Cancer Lett. 381, 23 (2016), Application(s): Western blotting and Immunoprecipitation, Abstract;
Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours: T. Golias, et al.; Sci. Rep. 6, 31146 (2016), Application(s): Western blotting antibodies, Abstract; Full Text
Metabolomic Analysis Reveals Increased Aerobic Glycolysis and Amino Acid Deficit in a Cellular Model of Amyotrophic Lateral Sclerosis: G.N. Valbuena, et al.; Mol. Neurobiol. 53, 2222 (2016), Application(s): Western Blot, Abstract; Full Text
Overcoming cisplatin resistance of ovarian cancer cells by targeting HIF-1-regulated cancer metabolism: Z. Ai, et al.; Cancer Lett. 373, 36 (2016), Application(s): Western blot, Abstract; Full Text
Methylation-dependent regulation of hypoxia inducible factor-1 alpha gene expression by the transcription factor kaiso: C.C. Pierre, et al.; Biochim. Biophys. Acta 1849, 1432 (2015), Application(s): Immunoblot analysis, Abstract;
pVHL is a regulator of glucose metabolism and insulin secretion in pancreatic β cells: W. Krek, et al. ; Genes Dev. 22, 3135 (2008), Application(s): WB using mouse cell lysates, Abstract;
Dichloroacetate, a metabolic modulator, prevents and reverses chronic hypoxic pulmonary hypertension in rats: role of increased expression and activity of voltage-gated potassium channels: S.L. Archer, et al. ; Circulation 105, 244 (2002), Application(s): WB using rat samples, Abstract;