The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
61kD
GeneID（Human）:
5551
Human Swiss-Prot No:
P14222
Cellular localization:
Cytolytic granule . Secreted. Cell membrane ; Multi-pass membrane protein . Endosome lumen . Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786). .
Background:
The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
Western Blot analysis of Hela cells using Perforin 1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200