PKC  Alpha Polyclonal Antibody

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCA (protein kinase C alpha) is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

The PKC Alpha Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of protein kinase C alpha (PKCα) in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human PKCα. The resulting polyclonal antibody exhibits high affinity and specificity towards PKCα, making it an ideal tool for various applications such as western blotting, immunohistochemistry, and immunofluorescence.

The PKC Alpha Polyclonal Antibody is validated for use in human, mouse, and rat samples, and has been extensively tested to ensure consistent and reliable results. This antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is recommended to store the antibody at -20°C for long-term storage and to avoid repeated freeze-thaw cycles.

Overall, the PKC Alpha Polyclonal Antibody is a valuable tool for researchers studying PKCα and its role in various biological processes. Its high specificity and sensitivity make it an ideal choice for detecting PKCα in complex biological samples, and its validated performance ensures reliable and consistent results.