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ELK Biotechnology
SKU(재고 관리 코드):ES5004
POLR3H Rabbit Polyclonal Antibody
POLR3H Rabbit Polyclonal Antibody
POLR3H Rabbit Polyclonal Antibody
Overview
| Product name: | POLR3H rabbit pAb |
| Alternative Names: | POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RPC8. AA range:151-200 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 23kD |
| GeneID(Human): | 171568 |
| Human Swiss-Prot No: | Q9Y535 |
| Cellular localization: | Nucleus. |
| Background: | function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.,similarity:Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex., |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of K562 cells using POLR3H Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from K562 cells, using RPC8 Antibody. The lane on the right is blocked with the synthesized peptide.
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