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Cloud-clone

SKU(재고 관리 코드):PAS097Hu01

Polyclonal Antibody to Actin (ACTIN)

Polyclonal Antibody to Actin (ACTIN)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

PAS097Hu01

Organism Species

Homo sapiens (Human) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

PAS097Hu01-Polyclonal Antibody to Actin (ACTIN)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ACTIN. It has been selected for its ability to recognize ACTIN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Molecular and Cellular Biochemistry Influence of signaling kinases on functional dynamics of nuclear receptor CAR Pubmed: 31352609
BMC Neurosci Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson's disease mouse model 34736417
Life Sci Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus Pubmed:34990649
Cell Signal Suppression of COX-2/PGE2 levels by carbazole-linked triazoles via modulating methylglyoxal-AGEs and glucose-AGEs–Induced ROS/NF-κB signaling in monocytes Pubmed:35640822
Free Radical Biology and Medicine Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH Pubmed:35697292
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
PAS097Hu01 Polyclonal Antibody to Actin (ACTIN) WB; IHC; ICC; IP.
MAS097Hu21 Monoclonal Antibody to Actin (ACTIN) WB; IHC; ICC; IP.
AES097Hu ELISA Kit for Anti-Actin Antibody (Anti-ACTIN) Enzyme-linked immunosorbent assay for Antibody Detection.

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