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SKU(재고 관리 코드):PAB908Hu02

Polyclonal Antibody to Aggrecan (AGC)

Polyclonal Antibody to Aggrecan (AGC)

UOM
Concentration
Organism species

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Product No.

PAB908Hu02

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB908Hu02-Recombinant Aggrecan (AGC)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.46mg/ml

Organism Species More

n/a

Applications

WB

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against AGC. It has been selected for its ability to recognize AGC in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Experimental Biology and Medicine

Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source

Age( dordr).

In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source

PLoS ONE

A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source

Stem Cell Research & Therapy

Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Pubmed: 24739658

Materials Science and Engineering: C

Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition ScienceDirect: S0928493113006656

PLoS One.

Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592

Connective Tissue Research

Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747

Macromolecular Bioscience

Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871

Osteoarthritis and Cartilage

Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs Pubmed:26802547

Cell Proliferation

Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063

bmc musculoskeletal disorders

Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731

Journal of Clinical Medicine

Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors Pubmed: 32392807


Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs

researchsquare

Aggrecan-a new biomarker for acute type A aortic dissection 33990642


Catalog No.

Related products for research use of Homo sapiens (Human) Organism species

Applications (RESEARCH USE ONLY!)

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ELISA Kit DIY Materials for Aggrecan (AGC)

Main materials for "Do It(ELISA Kit) Yourself"

 

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