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Cloud-clone

SKU(재고 관리 코드):PAB472Ra01

Polyclonal Antibody to Alkaline Phosphatase (ALP)

Polyclonal Antibody to Alkaline Phosphatase (ALP)

UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAB472Ra01

Organism Species

Rattus norvegicus (Rat) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ALP. It has been selected for its ability to recognize ALP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Clinical Oral Implants Research Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications Wiley: source
Die Pharmazie - An International Journal of Pharmaceutical Sciences The role of atorvastatin in bone metabolism in male albino Wistar rats Ingenta: art00010
22 Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135
Biomed Mater.? Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering Pubmed:24770944
Journal of Colloid and Interface Science Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198
Bone The active role of osteoporosis in the interaction between osteoblasts and bone metastases PubMed: 26057367
Macromolecular Bioscience Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665
journal of hard Tissue Biology Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb
Colloids Surf B Biointerfaces Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation PubMed: 26445021
Gels On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1
Acta Biomater Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470
Sci Rep A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration PubMed: 26632447
Acta Biomaterialia Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470
PLoS One. Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
J Cell Physiol. An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358
Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752
Journal of Inorganic Biochemistry Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953
Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Journal of Clinical Periodontology  Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Pubmed:29574908
Macromolecular Bioscience Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response Pubmed:29877029
Journal of Inorganic Biochemistry Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response Pubmed:29525694
DNA and Cell Biology miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3′ UTR of Pubmed:29359964
Bone The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats Pubmed: 30208342
Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats Pubmed: 30292771
BMC Complementary and Alternative Medicine  Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910
Journal of Hematology & Oncology Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment … Pubmed: 30621731
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization Pubmed: 30689288
Chemico-Biological Interactions Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080
Journal of Orthopaedic Surgery and Research Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
Bangladesh Journal of Medical Science Zn as the Factor of Alkaline Phosphatase in Periodontal Patients consuming Minangkabau food in West Sumatra, Indonesia
Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209
Journal of Korean Neurosurgical Society Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy Pubmed: 32172552
Journal of Applied Biomaterials & Functional Materials Effects of tricalcium silicate/sodium alginate/calcium sulfate hemihydrate composite cements on osteogenic performances in vitro and in vivo Pubmed: 32138579
Nutrients Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats Pubmed: 32668691
International Journal of Clinical and Experimental Pathology Matrilin-2 is a novel prognostic marker in osteosarcoma
Revista Argentina de Clínica Psicológica Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene
МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И …
Ceramics International Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response
Bioelectromagnetics Static Magnetic Field (2¨C4 T) Improves Bone Microstructure and Mechanical Properties by Coordinating Osteoblast/Osteoclast Differentiation in Mice 33655538
PeerJ From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling … Pubmed:35186473
Cell Death Discov ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway Pubmed:35136027
Int J Mol Sci Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677
Journal of Bionic Engineering Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
PAB472Ra01 Polyclonal Antibody to Alkaline Phosphatase (ALP) WB; IHC; ICC; IP.
SEB472Ra ELISA Kit for Alkaline Phosphatase (ALP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB472Ra Magnetic Luminex Assay Kit for Alkaline Phosphatase (ALP) ,etc. Magnetic Luminex Assay for Antigen Detection.


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