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Cloud-clone

SKU(재고 관리 코드):PAA457Ra01

Polyclonal Antibody to Caspase 14 (CASP14)

Polyclonal Antibody to Caspase 14 (CASP14)

UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAA457Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CASP14. It has been selected for its ability to recognize CASP14 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Dermatological Science Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis Pubmed:25315296
In Vitro Cell Dev Biol Anim Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes PubMed: 26427706
Applied Biochemistry and Biotechnology Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14 Pubmed: 30390204
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA457Ra01 Recombinant Caspase 14 (CASP14) Positive Control; Immunogen; SDS-PAGE; WB.
PAA457Ra01 Polyclonal Antibody to Caspase 14 (CASP14) WB; IHC; ICC; IP.
MAA457Ra21 Monoclonal Antibody to Caspase 14 (CASP14) WB; IHC; ICC; IP.
SEA457Ra ELISA Kit for Caspase 14 (CASP14) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA457Ra Magnetic Luminex Assay Kit for Caspase 14 (CASP14) ,etc. Magnetic Luminex Assay for Antigen Detection.


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