Cloud-clone
SKU(재고 관리 코드):PAA350Ra01
Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA350Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA350Ra01-Recombinant Collagen Type I Alpha 1 (COL1a1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against COL1a1. It has been selected for its ability to recognize COL1a1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Journal of Biomedical Materials | Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study Wiley: source |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847 |
| J Voice. | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429 |
| Arch Oral Biol. | Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Pubmed: 24370188 |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625 |
| UNIVERSA MEDICINA | Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture Univmed:Source |
| International Journal of Neuropsychopharmacology | The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. pubmed:27477355 |
| National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396 |
| Nephron. | Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor pubmed:28768285 |
| Cell and Tissue Research | Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and … Pubmed:29637306 |
| International Journal of Molecular Medicine | LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway Pubmed: 30320382 |
| International Journal of Molecular Sciences | Association between Plasma HMGB-1 and Silicosis: A Case-Control Study Pubmed: 30558126 |
| Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945 |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response |
| Scientific reports | Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells 33911160 |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA350Ra01 | Recombinant Collagen Type I Alpha 1 (COL1a1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA350Ra01 | Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) | WB; IHC; ICC; IP. |
| MAA350Ra21 | Monoclonal Antibody to Collagen Type I Alpha 1 (COL1a1) | WB; IHC; ICC; IP. |
| SEA350Ra | ELISA Kit for Collagen Type I Alpha 1 (COL1a1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA350Ra | Magnetic Luminex Assay Kit for Collagen Type I Alpha 1 (COL1a1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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