Cloud-clone
SKU(재고 관리 코드):PAA215Bo01
Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA215Bo01
Organism Species
Bos taurus; Bovine (Cattle).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against COL1a2. It has been selected for its ability to recognize COL1a2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Plos one | Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499 |
| J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731 |
| PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955 |
| American Journal of Translational Research | A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441 |
| Sci Rep | The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768 |
| Experimental and Therapeutic Medicine | Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847 |
| Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA215Bo01 | Recombinant Collagen Type I Alpha 2 (COL1a2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA215Bo01 | Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; IHC; ICC; IP. |
| MAA215Bo21 | Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2) | WB; IHC; ICC; IP. |
| SEA215Bo | ELISA Kit for Collagen Type I Alpha 2 (COL1a2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA215Bo | Magnetic Luminex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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