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SKU(재고 관리 코드):PAA215Ca01

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)

Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)

UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAA215Ca01

Organism Species

Canis familiaris; Canine (Dog).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA215Ca01-Recombinant Collagen Type I Alpha 2 (COL1a2)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL1a2. It has been selected for its ability to recognize COL1a2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Plos one Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731
PLoS One Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955
American Journal of Translational Research A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441
Sci Rep The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768
Experimental and Therapeutic Medicine Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA215Ca01 Recombinant Collagen Type I Alpha 2 (COL1a2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA215Ca01 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC; IP.
MAA215Ca21 Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC; IP.
SEA215Ca ELISA Kit for Collagen Type I Alpha 2 (COL1a2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA215Ca Magnetic Luminex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. Magnetic Luminex Assay for Antigen Detection.


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