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SKU(재고 관리 코드):PAA149Hu01

Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1)

Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAA149Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA149Hu01-Recombinant Collagen Type IV Alpha 1 (COL4a1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL4a1. It has been selected for its ability to recognize COL4a1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Cancer Research Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix–Directed Spheroid Formation Pubmed:26744523
PLoS One miR-146a regulates glucose induced upregulation of inflammatory cytokines extracellular matrix proteins in the retina and kidney in diabetes 10.1371
Journal of Diabetes & Metabolic Disorders Endothelin-1 traps potently reduce pathologic markers back to basal levels in an in vitro model of diabetes
Experimental and Molecular Pathology  Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909
Journal of Diabetes & Metabolic Disorders In vivo studies demonstrate that endothelin-1 traps are a potential therapy for type I diabetes
Frontiers in Endocrinology Curcumin Analogs Reduce Stress and Inflammation Indices in Experimental Models of Diabetes Pubmed: 31920992
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA149Hu01 Recombinant Collagen Type IV Alpha 1 (COL4a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA149Hu01 Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) WB; IHC; ICC; IP.
LAA149Hu71 Biotin-Linked Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) WB; IHC; ICC.
LAA149Hu81 FITC-Linked Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) WB; IHC; ICC; IF.
MAA149Hu22 Monoclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) WB; IHC; ICC; IP.
SEA149Hu ELISA Kit for Collagen Type IV Alpha 1 (COL4a1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA149Hu Magnetic Luminex Assay Kit for Collagen Type IV Alpha 1 (COL4a1) ,etc. Magnetic Luminex Assay for Antigen Detection.

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