Cloud-clone
SKU(재고 관리 코드):PAA149Ra01
Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1)
Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1)
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA149Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against COL4a1. It has been selected for its ability to recognize COL4a1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cancer Research | Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular MatrixâDirected Spheroid Formation Pubmed:26744523 |
| PLoS One | miR-146a regulates glucose induced upregulation of inflammatory cytokines extracellular matrix proteins in the retina and kidney in diabetes 10.1371 |
| Journal of Diabetes & Metabolic Disorders | Endothelin-1 traps potently reduce pathologic markers back to basal levels in an in vitro model of diabetes |
| Experimental and Molecular Pathology | Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909 |
| Journal of Diabetes & Metabolic Disorders | In vivo studies demonstrate that endothelin-1 traps are a potential therapy for type I diabetes |
| Frontiers in Endocrinology | Curcumin Analogs Reduce Stress and Inflammation Indices in Experimental Models of Diabetes Pubmed: 31920992 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA149Ra01 | Recombinant Collagen Type IV Alpha 1 (COL4a1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA149Ra01 | Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) | WB; IHC; ICC; IP. |
| MAA149Ra21 | Monoclonal Antibody to Collagen Type IV Alpha 1 (COL4a1) | WB; IHC; ICC; IP. |
| SEA149Ra | ELISA Kit for Collagen Type IV Alpha 1 (COL4a1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA149Ra | Magnetic Luminex Assay Kit for Collagen Type IV Alpha 1 (COL4a1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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