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SKU(재고 관리 코드):PAA387Mu01

Polyclonal Antibody to Complement Component 3a (C3a)

Polyclonal Antibody to Complement Component 3a (C3a)

UOM
Concentration
Organism species

Western blotting: 0.5-5µg/mL
;Immunohistochemistry: 5-50µg/mL
;Immunocytochemistry: 5-50µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

PAA387Mu01

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA387Mu01-Recombinant Complement Component 3a (C3a)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against C3a. It has been selected for its ability to recognize C3a in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-5µg/mL
Immunohistochemistry: 5-50µg/mL
Immunocytochemistry: 5-50µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

22

Immobilization of soluble complement receptor 1 on islets. ScienceDirect: S0142961211002596

Journal of Reproductive Immunology

Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845

Journal of Antimicrobial Chemotherapy

Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587

SpringerPlus

Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977

Gynecol Obstet Invest

Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247

Am J Reprod Immunol

Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study PubMed: 26388133

Journal of Neuroimmunology

Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358

Plos One

Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice Pubmed:26872035

Digital Repository

Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183

Colloids Surf B Biointerfaces.

Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns. pubmed:28131092

Pediatric Pulmonology

Accuracy of complement activation product levels to detect infected pleural effusion in rats pubmed:28474458

Journal of Immunology

The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression Pubmed:29367209

Artificial Cells Nanomedicine and Biotechnology Journal

Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly … Pubmed:29873524

Cytotherapy

Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation Doi: 10.1016/j.jcyt.2018.07.007

Journal of NeuroVirology

Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976

Clinical Immunology

Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss

Materials Science and Engineering: C

Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys

Nanomedicine

Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion 33549818

EXPERIMENTAL EYE RESEARCH

Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression 33493474

FASEB J

C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088


Catalog No.

Related products for research use of Mus musculus (Mouse) Organism species

Applications (RESEARCH USE ONLY!)

EPA387Mu61

Eukaryotic Complement Component 3a (C3a)

Positive Control; Immunogen; SDS-PAGE; WB.

RPA387Mu01

Recombinant Complement Component 3a (C3a)

Positive Control; Immunogen; SDS-PAGE; WB.

APA387Mu61

Active Complement Component 3a (C3a)

Cell culture; Activity Assays.

PAA387Mu01

Polyclonal Antibody to Complement Component 3a (C3a)

WB; IHC; ICC; IP.

SEA387Mu

ELISA Kit for Complement Component 3a (C3a)

Enzyme-linked immunosorbent assay for Antigen Detection.

HEA387Mu

High Sensitive ELISA Kit for Complement Component 3a (C3a)

Enzyme-linked immunosorbent assay for Antigen Detection.

LMA387Mu

Magnetic Luminex Assay Kit for Complement Component 3a (C3a) ,etc.

Magnetic Luminex Assay for Antigen Detection.

KSA387Mu01

ELISA Kit DIY Materials for Complement Component 3a (C3a)

Main materials for "Do It (ELISA Kit) Yourself".

 

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