SKU(재고 관리 코드):PAA017Hu02
Polyclonal Antibody to E-cadherin
Polyclonal Antibody to E-cadherin
Western blotting: 0.5-2µg/mL;1:170-680
Immunohistochemistry: 5-20µg/mL;1:17-68
Immunocytochemistry: 5-20µg/mL;1:17-68
Optimal working dilutions must be determined by end user.
Product No.
PAA017Hu02
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA017Hu01-Recombinant E-cadherin
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against E-cadherin. It has been selected for its ability to recognize E-cadherin in immunohistochemical staining and western blotting.
USAGE
Western blottin:g: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine |
Citations |
J Periodontal Res. |
Novel method for proliferation of oral keratinocyte stem cells Pubmed: 24329011 |
The Journal of Gene Medicine |
Modulation of the Dual‐Faced Effects of miR‐141 with Chitosan/miR‐141 Nanoplexes in Breast Cancer Cells Pubmed: 31389101 |
Cell Adhesion & Migration |
BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin Pubmed: 31957537 |
DOKLADY BIOCHEMISTRY AND BIOPHYSICS |
Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … Pubmed: 32342301 |
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … |
Catalog No. |
Related products for research use of Homo sapiens (Human) Organism species |
Applications (RESEARCH USE ONLY!) |
RPA017Hu01 |
Positive Control; Immunogen; SDS-PAGE; WB. |
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APA017Hu61 |
Cell culture; Activity Assays. |
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EPA017Hu61 |
Positive Control; Immunogen; SDS-PAGE; WB. |
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PAA017Hu01 |
WB; IHC; ICC; IP. |
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PAA017Hu02 |
WB; IHC; ICC; IP. |
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LAA017Hu71 |
WB; IHC; ICC. |
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LAA017Hu81 |
WB; IHC; ICC; IF. |
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MAA017Hu23 |
WB; IHC; ICC; IP. |
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MAA017Hu22 |
WB; IHC; ICC; IP. |
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MAA017Hu21 |
WB; IHC; ICC; IP. |
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MAA017Hu24 |
WB; IHC; ICC; IP. |
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SEA017Hu |
Enzyme-linked immunosorbent assay for Antigen Detection. |
|
SCA017Hu |
Chemiluminescent immunoassay for Antigen Detection. |
|
LMA017Hu |
Magnetic Luminex Assay for Antigen Detection. |