SKU(재고 관리 코드):PAA028Hu01
Polyclonal Antibody to Erythropoietin (EPO)
Polyclonal Antibody to Erythropoietin (EPO)
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA028Hu01
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA028Hu01-Recombinant Erythropoietin (EPO)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine | Citations |
Endocrinology | Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring Endo: source |
Journal of Ethnopharmacology | Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression PubMed: 21333724 |
PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source |
The Journal of Clinical Endocrinology & Metabolism | Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation PubMed: 22948762 |
Experimental & Molecular Medicine | Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression PubMed: PMC3509184 |
Biochem Biophys Res Commun. | Reevaluation of erythropoietin production by the nephron Pubmed:24832733 |
Nutrition. | Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. Pubmed:2528040 |
J Appl Physiol (1985). | Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness Pubmed:25324511 |
Expert Rev Proteomics | Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone PubMed: 26496240 |
Oncotarget | KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin pubmed:26575329 |
international journal of molecular sciences | Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. pubmed:28216608 |
Veterinary Clinical Pathology | Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease pubmed:28186645 |
IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease |
Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463 |
PLoS Neglected Tropical Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis pubmed:28489854 |
Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia S0197018617304783 |
Pesquisa Veterinária Brasileira | Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease 10.1590/s0100-736x2017000600011 |
Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation 10.1016/j.mjafi.2017.04.003 |
Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on … Pubmed:29180303 |
Clinical and Experimental Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502 |
Bulletin of Experimental Biology and Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565 |
Bulletin of Experimental Biology and Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … Pubmed: 30353332 |
FASEB JOURNAL | High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans |
Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats |
博士论文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE |
Nutrition | Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind … Pubmed: 31743809 |
BRITISH JOURNAL OF HAEMATOLOGY | Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans Pubmed: 32866306 |
JCI Insight. | Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes Pubmed: 32759495 |
Blood Cells, Molecules, and Diseases | EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness Pubmed: 32470757 |
Int J Endocrinol | miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia 34367277 |
J Mater Sci Mater Med | Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules Pubmed:35072831 |
Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
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