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SKU(재고 관리 코드):PAA437Hu01

Polyclonal Antibody to Estrogen Receptor Beta (ERb)

Polyclonal Antibody to Estrogen Receptor Beta (ERb)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Product No.

PAA437Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA437Hu01-Recombinant Estrogen Receptor Beta (ERb)

Buffer Formulation

PBS, pH7.4.

Traits

Freeze-dried powder

Concentration

1mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ERb. It has been selected for its ability to recognize ERb in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling Pubmed:24846829
Biochem Biophys Res Commun Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas PubMed: 26529545
Mol Neurobiol Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia PubMed: 26476840
J Steroid Biochem Mol Biol Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling PubMed: 24846830
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts science:S1001074216300924
Biol Open. Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine. pubmed:27875242
Biomedicine & Pharmacotherapy Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY Pubmed:29807224
Journal of Applied Pharmaceutical Science Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract Pbulication:325682424
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
Life Sciences MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl 34273377
Cardiometry Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA437Hu01 Recombinant Estrogen Receptor Beta (ERb) Positive Control; Immunogen; SDS-PAGE; WB.
PAA437Hu01 Polyclonal Antibody to Estrogen Receptor Beta (ERb) WB; IHC; ICC; IP.
LAA437Hu71 Biotin-Linked Polyclonal Antibody to Estrogen Receptor Beta (ERb) WB; IHC; ICC.
LAA437Hu81 FITC-Linked Polyclonal Antibody to Estrogen Receptor Beta (ERb) WB; IHC; ICC; IF.
MAA437Hu22 Monoclonal Antibody to Estrogen Receptor Beta (ERb) WB; IHC; ICC; IP.
MAA437Hu21 Monoclonal Antibody to Estrogen Receptor Beta (ERb) WB; IHC; ICC; IP.
SEA437Hu ELISA Kit for Estrogen Receptor Beta (ERb) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA437Hu CLIA Kit for Estrogen Receptor Beta (ERb) Chemiluminescent immunoassay for Antigen Detection.
LMA437Hu Magnetic Luminex Assay Kit for Estrogen Receptor Beta (ERb) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA437Hu01 ELISA Kit DIY Materials for Estrogen Receptor Beta (ERb) Main materials for "Do It (ELISA Kit) Yourself".

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