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SKU(재고 관리 코드):PAA037Hu02

Polyclonal Antibody to Fibronectin (FN)

Polyclonal Antibody to Fibronectin (FN)

UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Product No.

PAA037Hu02

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA037Hu02-Recombinant Fibronectin (FN)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

American Journal of Physiology - Renal Physiology

Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase PubMed: 19019916

Stem Cell Research Therapy

Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658

Molecules.

Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. Pubmed: 24013407

Journal of Ethnopharmacology

Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy ScienceDirect: S0378874114002414

Investigative Ophthalmology & Visual Science

Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells Pubmed: 24609629

PLoS One.

Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592

Connective Tissue Research

Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747

Laryngoscope.

Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625

Biomedical Materials

In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study Pubmed:25634132

Biomedical Materials

In vivo PubMed: 25634132

American Journal of Medical and Biological Research

Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease Ajmbr: 3

Food Chem.

Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk Pubmed:27173528

journal of perinatal medicine

Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. pubmed:27865093

Chinese Journal of Contemporary Neurology and Neurosurgery

Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke Article_en:CJFDTotal-XDJB201611014

Scientific Reports

Serum fbronectin distinguishes the early stages of hepatocellular carcinoma pubmed:28842594

Molecular Medicine Reports

Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression 10.3892/mmr.2017.7563

Cell Death & Disease

Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing. pubmed:29022912

Journal of Cellular Biochemistry

Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits Pubmed:29932250

Biomedicine & Pharmacotherapy

Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities Pubmed: 30530231

Advanced Healthcare Materials

Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809

Inflammation

TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling Pubmed: 31111299

EBioMedicine

Three kinds of corneal host cells contribute differently to corneal neovascularization

Renal Failure

Effects of autophagy on macrophage adhesion and migration in diabetic nephropathy Pubmed: 31352855

Journal of Biological Chemistry

Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328

Neurotherapeutics

Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair Pubmed: 31758411

FASEB Journal

Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis Pubmed: 31930571

Journal of Clinical Medicine

Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort Pubmed: 32210109

International Journal of Women¡¯s Health and Reproduction Sciences

Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women?


Advanced glycation end-products associate with podocytopathy in type II diabetic patients

IBRO Neuroscience Reports

Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function Pubmed:35586775


Catalog No.

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Applications (RESEARCH USE ONLY!)

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ELISA Kit DIY Materials for Fibronectin (FN)

Main materials for "Do It (ELISA Kit) Yourself".

 

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